Module 1 of the Basic and Applied Cytometry Course aims to introduce PhD students to Applied Flow Cytometry. We want to illustrate not only the general principles of this investigation technique, fundamental in both clinical and research fields, but also its many applications, so that the doctoral student can evaluate its possible use in the context of his own research project and have way to follow in detail the use in the immunological, oncological, ecotoxicological fields, and related to the use of nanostructures. Module 1 is to be considered closely associated with Mod.2 (Prof S. Papa) in which the circuit parts (and the physical bases) will be deepened.

The generalities of the basic principles of the technology (Optical, Fluidic and Electronic System), fluorescence, direct and indirect immunofluorescence methods, the appropriate reagentarium of the flow cytometry laboratory, and the type of samples suitable for this analytical approach will be briefly summarized . Subsequently, various cytometric applications will be discussed not only in the field of clinic and research applied to humans, but also in the veterinary and microbiological fields from a One Health perspective, with a focus on cells of various organisms useful as bioindicators, with evidence of the approach flow cytometry. The module will conclude by focusing the cytometric analysis in the field of cellular functional analysis, with examples of cells taken from bioindicator organisms for their possible use as markers of environmental stress.

Module of Prof Stefano Papa

The student, once followed the course in its 2 modules, will be able to evaluate the possibility of using this technology and the possible benefits that the application of this investigation procedure can bring to his research project. Moreover, in particular in mod.1, the PhD student will be able to see and understand in detail, the immunological and environmental applications, of flow cytometry.

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

By agreement with the PhD students, a lesson will be organized in the aboratorio of flow cytometry.

Teaching

lectures and a possible exercise

Course books

No study texts are indicated for attending the module, however for those wishing to deepen, we recommend the text "Flow cytometer" by C. Ortolani, edi-ermes.