INSTRUMENTAL ANALYTICAL CHEMISTRY
CHIMICA ANALITICA STRUMENTALE
| A.Y. | Credits |
|---|---|
| 2015/2016 | 6 |
| Lecturer | Office hours for students | |
|---|---|---|
| Achille Cappiello | Appointment by email |
Assigned to the Degree Course
| Date | Time | Classroom / Location |
|---|
Learning Objectives
During the course of Analytical Chemistry the student will learn some of the most advanced analytical techniques. The most important instrumental methods will be covered as well, in particular those for modern and advanced biological applications. Technical details and applications will be also considered. Particular emphasis will be given to chromatography and mass spectometry.
Program
Units of measurement. Preparation of solutions and dilutions. Validation of analytical methods: The error of measurement: Significant figures. Errors and their detection and minimization. Precision and accuracy. Calibration. Pearson coefficient. Method of least squares. Standardization procedures. Chromatographic methods: distribution coefficient. Column chromatography. The chromatogram and the chromatographic parameters. Chromatographic resolution and efficiency. Band broadening. Van Deemter equation. Basics of gas chromatography. High performance liquid chromatography : stationary phases and mobile phases. Types of columns. UHPLC and other high efficiency techniques. Separation mechanisms: absorption, distribution, ion exchange, size exclusion. Ion chromatography. HPLC injector. HPLC detectors: RI, UV-Vis, fluorescence, electrochemical detectors.Mass Spectrometry: ionization techniques: electronic ionization, chemical ionization, electrospray ionization (ESI), matrix effects, multicharged ions, atmospheric pressure chemical ionization (APCI), Matrix-assisted Laser Desorption Ionization (MALDI). General features of a mass spectrum. Mass spectrometric resolution . Fragmentation characteristics. Isotopic cluster. Mass analyzer: quadrupole (Q), ion trap (QIT), time of flight (TOF). Combination of multiple analyzers, QQQ, Q-TOF, TOF / TOF, etc.. Tandem Mass Spectrometry (MS / MS) and collision ion dissociation (CID). Chromatography-mass spectrometry coupling, GC-MS and HPLC-MS. instrument configurations and operating modes. Matrix effects and their impact on complex samples. HPLC-MS interfaces . Using the mass spectrometer in the analytic field: exact mass, elemental analysis, SIM, SRM, MRM, Scan.
Teaching Material
The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it
Supporting Activities
Slides presentations.
Teaching, Attendance, Course Books and Assessment
- Teaching
Slides presentation.
- Attendance
Students who have attended at least 80% of lessons.
- Course books
Slides presentation, suggested booksi: Cozzi, Protti, Ruaro - Elementi di analisi chimica strumentale - Seconda edizione - Zanichelli, Holler, Skoog, Crouch - Chimica analitica strumentale - Seconda edizione - EdiSES, Dong - Modern HPLC for Practicing Scientists - Wiley, Dass - Fundamentals of Contemporary Mass Spectrometry - Wiley, Cappiello - Advances in LC-MS Instrumetation - Elsevier
- Assessment
Written test
- Disability and Specific Learning Disorders (SLD)
Students who have registered their disability certification or SLD certification with the Inclusion and Right to Study Office can request to use conceptual maps (for keywords) during exams.
To this end, it is necessary to send the maps, two weeks before the exam date, to the course instructor, who will verify their compliance with the university guidelines and may request modifications.
Additional Information for Non-Attending Students
- Attendance
Students who have attended less than 80% of lessons.
- Course books
Slides presentation, suggested books: Cozzi, Protti, Ruaro - Elementi di analisi chimica strumentale - Seconda edizione - Zanichelli, Holler, Skoog, Crouch - Chimica analitica strumentale - Seconda edizione - EdiSES, Dong - Modern HPLC for Practicing Scientists - Wiley, Dass - Fundamentals of Contemporary Mass Spectrometry - Wiley, Cappiello - Advances in LC-MS Instrumetation - Elsevier
- Assessment
Written test plus discussion of two scientific papers for those students who have attended less than 80% of lessons.
- Disability and Specific Learning Disorders (SLD)
Students who have registered their disability certification or SLD certification with the Inclusion and Right to Study Office can request to use conceptual maps (for keywords) during exams.
To this end, it is necessary to send the maps, two weeks before the exam date, to the course instructor, who will verify their compliance with the university guidelines and may request modifications.
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