During the course of Analytical Chemistry the student will learn some of the most advanced analytical techniques. The most important instrumental methods will be covered as well, in particular those for modern and advanced biological applications. Technical details and applications will be also considered. Particular emphasis will be given to chromatography, mass spectometry and spectroscopy.

Units of measurement. Preparation of solutions and dilutions. Validation of analytical methods: The error of measurement: Significant figures. Errors and their detection and minimization. Precision and accuracy. Calibration. Pearson coefficient. Method of least squares. Standardization procedures. Sample preparation techniques. Extraction and preconcentration. Recovery efficiency. Spectroscopic methods: properties of electromagnetic radiation and its interaction with matter. Instrumentation. Molecular absorption UV-visible: the Beer-Lambert law and its deviations. Molecular absorption in the region of UV-Vis. Qualitative and quantitative analysis. Methods of fluorescence. Inductive coupled plasma (ICP). Chromatographic methods: distribution coefficient. Column chromatography. The chromatogram and the chromatographic parameters. Chromatographic resolution and efficiency. Band broadening. Van Deemter equation. High performance liquid chromatography : stationary phases and mobile phases. Types of columns. UHPLC and other high efficiency techniques. Separation mechanisms: absorption, distribution, ion exchange, size exclusion. Ion chromatography. HPLC injector. HPLC detectors: RI, UV-Vis, fluorescence, electrochemical detectors.Mass Spectrometry: ionization techniques: electronic ionization, chemical ionization, electrospray ionization (ESI). General features of a mass spectrum. Mass spectrometric resolution. Fragmentation characteristics. Isotopic cluster. Mass analyzer: quadrupole (Q), ion trap (QIT), time of flight (TOF). Chromatography-mass spectrometry coupling, HPLC-MS. instrument configurations and operating modes. Using the mass spectrometer in the analytic field: SIM, Scan.

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

Course books

Slides, Suggested books: Cozzi, Protti, Ruaro - Elementi di analisi chimica strumentale - Seconda edizione - Zanichelli, Holler, Skoog, Crouch - Chimica analitica strumentale - Seconda edizione - EdiSES

Assessment

Written test with open questions.

Disability and Specific Learning Disorders (SLD)

Students who have registered their disability certification or SLD certification with the Inclusion and Right to Study Office can request to use conceptual maps (for keywords) during exams.

To this end, it is necessary to send the maps, two weeks before the exam date, to the course instructor, who will verify their compliance with the university guidelines and may request modifications.