Università degli Studi di Urbino Carlo Bo / Portale Web di Ateneo


INTERNSHIP:BIO-MOLECULAR TECHNIQUES IN THE STUDY OF MUSCLE CELLS C2C12 DURING THE DIFFERENTIATION PROCESS
TIROCINIO: TECNICHE BIO-MOLECOLARI NELLO STUDIO DELLE CELLULE MUSCOLARI C2C12 DURANTE IL PROCESSO DI DIFFERENZIAMENTO

A.Y. Credits
2021/2022 3
Lecturer Email Office hours for students
Deborah Agostini from Monday to Friday by appointment via email (deborah.agostini@uniurb.it)
Teaching in foreign languages
Course with optional materials in a foreign language English
This course is entirely taught in Italian. Study materials can be provided in the foreign language and the final exam can be taken in the foreign language.

Assigned to the Degree Course

Physical Education for Health and Prevention (LM-67)
Curriculum: PERCORSO COMUNE
Date Time Classroom / Location
Date Time Classroom / Location

Learning Objectives

The internship is designed to provide the student with basic knowledge on some of the techniques normally used in biochemistry and molecular biology. The primary objective is to illustrate to the students the techniques for the study of muscle cells in differentiation

Program

Introductory seminar on  muscle cell differentiation and on the techniques suitable for studying it.

In the laboratory: •

·  in vitro propagation of murine myoblasts

·  specific staining for cellular structures in myoblasts and myotubes

·  molecular biology techniques with particular attention to Real Time PCR for the study of gene expression

·  electrophoretic techniques for the proteome study

Learning Achievements (Dublin Descriptors)

The student must be able to describe, with appropriate terminology, the above techniques (myoblasts and myotubes propagation, specific staining, nucleic acid preparation, reverse transcription, PCR and Real time PCR). The laboratory activity will allow the student to experiment and concretely apply the described techniques. Above all, he should be able to critically evaluate how to deal with the study of cell differentiation

Teaching Material

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

Supporting Activities

Any material will be given during the course

Practical activities in the laboratory are planned


Didactics, Attendance, Course Books and Assessment

Didactics

lectures and activities in the laboratory

Attendance

obligation to attend classes and laboratories

Course books

Any material will be delivered during the course and / or uploaded to the moodle platform

Assessment

The evaluation will be carried out by preparing a written report. The student must demonstrate adequate knowledge of the biomolecular techniques described and applied during the internship. It will also critically evaluate the applicability of these techniques to meet specific research needs

« back Last update: 20/07/2021

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