The course aims to provide the student with an overview of microbial molecular diagnostic techniques, followed by an in-depth study of the techniques for detecting, quantifying and typing the Leishmania parasite.

part I

Targets of molecular diagnostics
Specimen collection
Sample preparation
Quality controls
Selection of target sequences 
Molecular detection of microorganisms 
Molecular detection of resistance to antimicrobial agents
Molecular typing methods for epidemiological studies
PFGE
RAPD
ITS sequence characterization
MLST

part II

Leishmania parasites
Genome organization
Molecular diagnostics (methods for detection and typing)
Real-time PCR (qPCR)
Molecular targets
Clinical samples
Approaches for detection/quantification and typing Leishmania species
 

D1 - Knowledge and ability of comprehension. The student must demonstrate to have gained the knowledge inherent to the teaching program.
D2 - Ability to apply knowledge and comprehension. The student must be able to implement/use these knowledges for the critical evaluation of the molecular diagnostic methods.
D3 - Autonomy of judgement. The student will be able to critically evaluate the diagnostic approaches for leishmaniasis.
D4 - Communication skills. The student must acquire adequate scientific language skills by taking a participatory attitude to the topics of the lectures.
D5 - Learning skill. The student will be autonomously able to deepen the topics covered.
 

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

Teaching

Frontal and on-line (zoom platform) lectures. The zoom link for the lecture will be available in the Moodle platform.

Assessment

Assessment will be carried out through multiple choice test.

Disability and Specific Learning Disorders (SLD)

Students who have registered their disability certification or SLD certification with the Inclusion and Right to Study Office can request to use conceptual maps (for keywords) during exams.

To this end, it is necessary to send the maps, two weeks before the exam date, to the course instructor, who will verify their compliance with the university guidelines and may request modifications.