Module 1 of the Basic and Applied Cytometry Course aims to introduce doctoral students to Flow Cytometry applied in particular to Immunology. We want to illustrate not only the general principles of this technique of investigation (essential in both clinical and research activities), but also its several applications, so that the student can assess the possible use in their research project and have the opportunity to follow in detail the deep application in immunology and oncology.

Module 1 is to be considered closely associated with Mod. 2 (Prof S. Papa) in which the circuit parts (and the physical basis) will be deepened.

In the first lesson will be presented the generalities of the basic principles of technology (Optical, Fluidic and Electronic System), fluorescence, direct and indirect immunofuorescence methods, the appropriate reagents of the flow cytometic lab, and the type of samples suitable for this analytical approach. In the second lesson, entitled "Myeloid cell deviation in cancer and Covid-19 ", myeloid cells with immunoregulatory functions will be defined and characterized, with evidence of the cytofluorometric approach in Oncology and Immunology. The last lecture of this module will focus on the cytometric analysis of Extracellular Vesicles, defining their biological significance, characterization and content (cargo), as well as their character of possible biomarker in some diseases

It is recommended to follow module 1 and module 2 at the same time

The student, once followed the course in its 2 modules, will be able to evaluate the possibility of the use of this technology and the possible benefits that the application of this procedure of investigation can bring to his research project. Moreover, the PhD student will have the opportunity to extend the knowledge to details of the clinical, immunological and oncological applications of flow cytometry (mod.1), starting from a research basis.

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

Upon agreement with the doctoral student, attendance at the cytometry lab will be possible.

Teaching

Lectures and possible individual exercises after the agreement with the individual doctoral student who may be interested in employing it in their project.

Attendance

At least 2 out of 3 lessons must be attende by the student

Course books

Study texts are not indicated for the attendance of the module, however, for those who wish to apply in depth flow cytometric protocols and technique, we recommend the text "Flow cytometry" by C. Ortolani, edi-ermes.

Assessment

A short quiz on the activities will be administered at the conclusion of the entire module.

Disability and Specific Learning Disorders (SLD)

Students who have registered their disability certification or SLD certification with the Inclusion and Right to Study Office can request to use conceptual maps (for keywords) during exams.

To this end, it is necessary to send the maps, two weeks before the exam date, to the course instructor, who will verify their compliance with the university guidelines and may request modifications.