The BIOTECHNOLOGY LABORATORY course aims to illustrate the fundamental analytical techniques and methods most relevant to the aims of the degree course.

The fundamental theoretical and applicative aspects of analytical laboratory practice will be explored in depth, with particular reference to classical methods while introducing modern techniques.

The course is divided into theoretical lessons and practical experiences.

1. Safety rules in the chemistry laboratory: a) General rules.

2. Equipment commonly used in the laboratory: a) Technical and analytical scale; b) glassware; c) vortex; stirring and heating plates; centrifuges, etc.

3. Measurement concept: a) Concepts of precision and accuracy; b) standard deviation; c) qualitative and quantitative analysis; d) validation of the measurement; e) targeted and non-targeted analysis.

4. Weight and volume measurements: a) Use of scales; b) volume measurements using glass and automatic pipettes.

5. The solutions: a) Solution concept; b) concentration of solutions; c) concentrated solution and dilution procedures; d) preparation of some solutions.

6. Calibration procedures: a) External standard; b) internal standard; c) standard additions

7. Titrations

8. Sample Preparation: a) Theory and practice of sampling. Nature of complex samples; b) Sample preparation: theoretical aspects and practical problems in the treatment of real samples and their conservation; c) liquid phase extraction techniques; d) solid phase extraction techniques; e) membrane-based extraction techniques; f) rapid and miniaturized techniques (MEPS, DLLME); g) innovative sample preparation methods: Solid Phase Micro Extraction, QuEChERS, Stir Bar Sorptive Extraction, passive samplers.

9. Spectroscopic techniques: a) electromagnetic waves; b) nature of light; c) absorption and emission; d) fluorescence and phosphorescence; e) principles of spectrophotometry.

D1: Knowledge and understanding. The student will have to learn the principles and use of the main analytical techniques available for the qualitative and quantitative determination of analytes. They must also know what the most characteristic and essential substances present in biological tissues are and how they are determined. The student must understand the techniques available for sampling, conservation and qualitative-quantitative analysis of chemical components and how to apply them to real samples.

D2: Ability to apply knowledge and understanding. The student must be able to use the terminology related to analytical chemistry correctly. You will have to master the units of measurement of concentration. They must be able to choose among the sampling, sample treatment, and analysis techniques most appropriate for analyzing biological samples. These skills will be tested through written questions.

D3: Independence of judgment. The student must be able to make the correct decisions on how to address a problem related to the analysis and evaluation of the data obtained.

D4: Communication skills. The student must be able to clearly describe the analytical techniques studied with appropriate terms and appropriate examples.

D5: Learning ability. The student's scientific growth must be critical and autonomous, correctly using the material provided by the teacher and what he finds independently to deepen his study.

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

Not foreseen.

Teaching

Lectures and practical exercises.

Attendance

Admission to the exam is permitted to students who have attended at least 2/3 of the laboratory hours and lectures, as attendance is compulsory. To be able to participate in the laboratories, it is mandatory to have followed and passed the final test of the General Training Course on Safety for Workers and the Course on Safety in the Chemical and Biological Laboratory.

Course books

Fundamental of Analytical Chemistry

Skoog & West.

Editor: Edises

Edition: 3

Date of publication: 2015

ISBN: 8879598651

Assessment

Written exam with a total of 6 open questions on different topics from the entire program.

There is a maximum time of one hour for the exam and a pre-printed form with space for six answers. The evaluation considers various aspects, including the number of correct answers and their quality.

Disabilità e DSA

Le studentesse e gli studenti che hanno registrato la certificazione di disabilità o la certificazione di DSA presso l'Ufficio Inclusione e diritto allo studio, possono chiedere di utilizzare le mappe concettuali (per parole chiave) durante la prova di esame.

A tal fine, è necessario inviare le mappe, due settimane prima dell’appello di esame, alla o al docente del corso, che ne verificherà la coerenza con le indicazioni delle linee guida di ateneo e potrà chiederne la modifica.

Teaching

Course with mandatory attendance.

Course books

Fundamental of Analytical Chemistry

Skoog & West.

Editor: Edises

Edition: 3

Date of publication: 2015

ISBN: 8879598651

Assessment

Written exam with a total of 6 open questions on different topics from the entire program.

There is a maximum time of one hour for the exam and a pre-printed form with space for six answers. The evaluation considers various aspects, including the number of correct answers and their quality.

Disabilità e DSA

Le studentesse e gli studenti che hanno registrato la certificazione di disabilità o la certificazione di DSA presso l'Ufficio Inclusione e diritto allo studio, possono chiedere di utilizzare le mappe concettuali (per parole chiave) durante la prova di esame.

A tal fine, è necessario inviare le mappe, due settimane prima dell’appello di esame, alla o al docente del corso, che ne verificherà la coerenza con le indicazioni delle linee guida di ateneo e potrà chiederne la modifica.

The slides will be provided before each lesson.