The course aims to provide theoretical and practical knowledge regarding the main methodologies (both classical and instrumental) for the quantitative determination of substances of pharmaceutical and toxicological interest with references to the Official Pharmacopoeia.

Introduction to the course and basic concepts Safety, general concepts of Quantitative pharmaceutical analysis.

Volumetric methods in quantitative analysis (acid-base, precipitation, complexometric and redox titrations), solubility, redox and complexation equilibria.

UV/visible spectroscopy - Instrumentation and sample preparation Quantitative analysis: Lambert-Beer law, applications of interest of the instrumental technique in quantitative analysis of substances of pharmaceutical and toxicological interest with references to the Official Pharmacopoeia.

Chromatographic techniques – Gas chromatography, HPLC: instrumentation. Stationary and mobile phases and detectors; parameters that regulate the performance of HPLC and applications of the instrumental technique in quantitative analysis of substances of pharmaceutical and toxicological interest with references to the Official Pharmacopoeia. Ultra-performance liquid chromatography (UPLC), thin layer chromatography (TLC).

The practical exercises, whose schedule will be communicated by the teacher at the beginning of the course, are focused on the analysis of substances of pharmaceutical and toxicological interest with references to the Official Pharmacopoeia.

To attend the Quantitative Pharmaceutical Analysis laboratory with laboratory, it is necessary to have passed the General and Inorganic Chemistry exam and to have attended Analytical Chemistry with laboratory.

D1- KNOWLEDGE AND UNDERSTANDING-

The student must have acquired theoretical and practical knowledge related to the main quantitative chemical analytical techniques, both classical and instrumental. Ability to understand the most recent analytical techniques in the quantitative dosage of active ingredients in variously complex systems, in drug stability controls and in the determination of the purity of a drug. These skills will be assessed through laboratory experiences.

D2-APPLYING KNOWLEDGE AND UNDERSTANDING

The student must demonstrate the ability to choose and apply the most appropriate quantitative analytical techniques for the determination of substances of pharmaceutical interest, belonging to the Official Pharmacopoeia, in more or less complex systems, obtaining valid and verified results. The student must also be able to program and apply an analytical method.

D3-AUTONOMY OF JUDGMENT

The student must demonstrate the ability to express a critical choice of the most appropriate analysis method autonomously, therefore independently, producing effective results. The student must also be able to interpret the experimental data and the results obtained. These skills will be verified through targeted oral questions of an applicative nature

D4-COMMUNICATION

At the end of the course the student must be able to present what he/she has learned using appropriate terminology for the purpose of correct scientific communication regarding methodologies and issues concerning the quantitative analysis of substances of pharmaceutical interest. He/she must also be able to organize and present clear and exhaustive reports.

D5-LIFELONG LEARNING SKILLS

 The student must demonstrate good autonomy in carrying out quantitative analyses of substances of pharmaceutical interest described in the Official Pharmacopoeia and possess the ability to carry out research in literature and/or online for alternative and innovative methods in order to maintain a high level of training.

The teaching material prepared by the lecturer in addition to recommended textbooks (such as for instance slides, lecture notes, exercises, bibliography) and communications from the lecturer specific to the course can be found inside the Moodle platform › blended.uniurb.it

No teaching support activities are planned.

Teaching

The course includes: lectures - individual/group participatory laboratory lessons

Innovative teaching methods

La modalità didattica laboratoriale verrà arricchita con esercitazioni e approfondimenti, individuali e di gruppo seguendo la pratica del “learning by doing”.

Attendance

Attendance at laboratory exercises is mandatory. To access the laboratory, it is necessary to have passed the exam in General and Inorganic Chemistry and to have attended Analytical Chemistry with laboratory.

Course books

Study texts:

D. G. Watson Analisi Farmaceutica, terza edizione, edra ed.

A. Carrieri, Manuale di Analisi quantitativa dei Medicinali, Ed. EdiSES

Consultation texts:

E. Abigante, D. Melisi, M.G. Rimoli, Principi di Analisi quantitativa dei Medicinali, Ed. Loghia

G.C. Porretta, Analisi di preparazioni Farmaceutiche, Ed. CISU

Assessment

The expected learning outcomes will be assessed through reports on practical laboratory experiences. The assessment of the aforementioned tests will have a formative character in order to test the mastery of knowledge and skills related to the application of Quantitative analysis on substances of pharmaceutical interest and the presentation of the data collected during the same. Subject to approval of the practical laboratory part (Prof. Retini), the assessment of learning includes an oral exam (Prof. Bernetti) aimed at verifying the student's acquisition of the theoretical concepts presented in class and the practical procedures used during the laboratory exercises. The student will be required to have knowledge regarding the classical and instrumental techniques used in the determination of the purity of drugs and in the quantitative analysis of active ingredients in more or less complex systems. The student's ability to connect the topics covered during the lessons as well as to solve practical and applicative problems will also be verified. The oral exam is judged with a grade in thirtieths.

Disability and Specific Learning Disorders (SLD)

Students who have registered their disability certification or SLD certification with the Inclusion and Right to Study Office can request to use conceptual maps (for keywords) during exams.

To this end, it is necessary to send the maps, two weeks before the exam date, to the course instructor, who will verify their compliance with the university guidelines and may request modifications.

Teaching

No recovery activities are planned.

Attendance

Attendance at laboratory exercises is mandatory. To access the laboratory, it is necessary to have passed the exam in General and Inorganic Chemistry and to have attended Analytical Chemistry with laboratory.

Course books

Study texts:

D. G. Watson Analisi Farmaceutica, terza edizione, edra ed.

A. Carrieri, Manuale di Analisi quantitativa dei Medicinali, Ed. EdiSES

Consultation texts:

E. Abigante, D. Melisi, M.G. Rimoli, Principi di Analisi quantitativa dei Medicinali, Ed. Loghia

G.C. Porretta, Analisi di preparazioni Farmaceutiche, Ed. CISU

Assessment

The expected learning outcomes will be assessed through reports on practical laboratory experiences. The assessment of the aforementioned tests will have a formative character in order to test the mastery of knowledge and skills related to the application of Quantitative analysis on substances of pharmaceutical interest and the presentation of the data collected during the same. Subject to approval of the practical laboratory part (Prof. Retini), the assessment of learning includes an oral exam (Prof. Bernetti) aimed at verifying the student's acquisition of the theoretical concepts presented in class and the practical procedures used during the laboratory exercises. The student will be required to have knowledge regarding the classical and instrumental techniques used in the determination of the purity of drugs and in the quantitative analysis of active ingredients in more or less complex systems. The student's ability to connect the topics covered during the lessons as well as to solve practical and applicative problems will also be verified. The oral exam is judged with a grade in thirtieths.

Disability and Specific Learning Disorders (SLD)

Students who have registered their disability certification or SLD certification with the Inclusion and Right to Study Office can request to use conceptual maps (for keywords) during exams.

To this end, it is necessary to send the maps, two weeks before the exam date, to the course instructor, who will verify their compliance with the university guidelines and may request modifications.